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Number of cell counts in the peripheral blood of normal volunteers and patients with stage IV CRC. A . Representative images of typical circulating tumor cells <t>(CTCs,</t> CK+/CD45−) and circulating hybrid cells (CHCs, CK+/CD45+ isolated by <t>the</t> <t>IsoFlux</t> system (right panel) and counted by imaging software: Gen5 3.08 (left panel); scale bar, 100 μm B the number of circulating tumor cells (CTCs, CK+/CD45−) p value = 0.045 C the number of circulating hybrid cells (CHCs, CK+/CD45+) p value= 0.029 D the number of tumor-associated circulating endothelial cells (rare cells) p value= 0.979; E the number of CTC, CHC and rare cells F representative images of CHC (CK+/ EpCAM+/CD45+) detected by a GeoMx® instrument; scale bars, 50 μm. The Mann–Whitney U test or the T test was used to compare whether there was a difference in the dependent variable between the two independent groups. Statistical significance was established at p value < 0.05
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Number of cell counts in the peripheral blood of normal volunteers and patients with stage IV CRC. A . Representative images of typical circulating tumor cells (CTCs, CK+/CD45−) and circulating hybrid cells (CHCs, CK+/CD45+ isolated by the IsoFlux system (right panel) and counted by imaging software: Gen5 3.08 (left panel); scale bar, 100 μm B the number of circulating tumor cells (CTCs, CK+/CD45−) p value = 0.045 C the number of circulating hybrid cells (CHCs, CK+/CD45+) p value= 0.029 D the number of tumor-associated circulating endothelial cells (rare cells) p value= 0.979; E the number of CTC, CHC and rare cells F representative images of CHC (CK+/ EpCAM+/CD45+) detected by a GeoMx® instrument; scale bars, 50 μm. The Mann–Whitney U test or the T test was used to compare whether there was a difference in the dependent variable between the two independent groups. Statistical significance was established at p value < 0.05

Journal: Clinical and Experimental Medicine

Article Title: Tumor-immune hybrid cells evade the immune response and potentiate colorectal cancer metastasis through CTLA4

doi: 10.1007/s10238-024-01515-9

Figure Lengend Snippet: Number of cell counts in the peripheral blood of normal volunteers and patients with stage IV CRC. A . Representative images of typical circulating tumor cells (CTCs, CK+/CD45−) and circulating hybrid cells (CHCs, CK+/CD45+ isolated by the IsoFlux system (right panel) and counted by imaging software: Gen5 3.08 (left panel); scale bar, 100 μm B the number of circulating tumor cells (CTCs, CK+/CD45−) p value = 0.045 C the number of circulating hybrid cells (CHCs, CK+/CD45+) p value= 0.029 D the number of tumor-associated circulating endothelial cells (rare cells) p value= 0.979; E the number of CTC, CHC and rare cells F representative images of CHC (CK+/ EpCAM+/CD45+) detected by a GeoMx® instrument; scale bars, 50 μm. The Mann–Whitney U test or the T test was used to compare whether there was a difference in the dependent variable between the two independent groups. Statistical significance was established at p value < 0.05

Article Snippet: Initially, 10 ml of peripheral whole blood samples in EDTA tube from stage IV CRC patients ( N = 10) and healthy controls ( N = 10) were processed using the CTCs enrichment kit from the IsoFlux liquid biopsy system (Fluxion Biosciences, CA, USA), which is based on EpCAM expression [ ].

Techniques: Isolation, Imaging, Software, MANN-WHITNEY